Infections are the main reason for the high rates of patient mortality and morbidity in developing nations like Pakistan. Operating Theatres (OTs) are the main hospital areas where strict adherence to infection control protocols is required. Standard precautions, which are based on good practices, adequate knowledge, and healthy self-care practices, can lessen the impact of illnesses.

To assess the knowledge, attitude and practice of OT professionals regarding infection control protocols.

A cross-sectional study was conducted in PINS Hospital, Lahore. Ethical approval from the institutional review board committee was obtained. A well-structured questionnaire was used to gather data. A total of 104 healthcare professionals, including OT technologists, nurses, anaesthesiologists, surgeons, house officers, OT managers and sweepers, were chosen using a simple random sampling technique. Statistical association between variables was examined using the chi-square test. Data interpretation was conducted using SPSS version 27.

Knowledge, attitude and practice among 104 healthcare workers were assessed. Of the 104 participants, 5, 89 and 10 demonstrated high, moderate and low awareness of infection control protocols, respectively. Regarding practice, 45 healthcare workers demonstrated a moderate practice rate, 10 demonstrated a high practice rate, and 49 demonstrated a poor practice rate.

According to the study findings, staff nurses and sweepers expressed a low level of infection-control knowledge and practice, while surgeons demonstrated the highest level.